Journal: BMC Veterinary Research

Article Title: Effects of artichoke ( Cynara scolymus ) polyphenolic extract on growth, antioxidants and some immune parameters in common carp ( Cyprinus carpio )

doi: 10.1186/s12917-025-04619-w

Figure Lengend Snippet: Effect of different levels of ALE on serum antioxidant enzymes activity of C. Carpio

Article Snippet: Employing a commercial kit, the serum activity of catalase (CAT) (ZellBio GmbH, Germany, E-BC-K031-S), superoxide dismutase (SOD), (ZellBio GmbH, Germany, ZX-44108-96) and glutathione peroxidase (GPx) (ZellBio GmbH, Germany, E-BC-K096-M) was measured according to the manufacturer’s protocol [ ].

Techniques: Activity Assay

Journal: Turkish Journal of Chemistry

Article Title: Synthesis and characterization of new hexahydroquinoline derivatives and evaluation of their cytotoxicity, intracellular ROS production, and inhibitory effects on inflammatory mediators

doi: 10.55730/1300-0527.3686

Figure Lengend Snippet: Inflammation markers and and complement protein levels in the study groups. IL-1α: interleukin-1-alpha; IL-10: interleukin-10; TGF-β1: transforming growth factor-1-beta; TNF-α: tumor necrosis factor-alpha; C3: complement C3; C9: complement C9.

Article Snippet: TGF-β1, IL-1a, IL-10, TNF-α, C3, and C9 kits were purchased from BT Lab (Birmingham, UK).

Techniques:

Journal: RSC Advances

Article Title: Design, synthesis, and mechanistic evaluation of novel pyrazole/thiazole chalcone hybrids as dual tubulin polymerization and COX-2 inhibitors with potent antiproliferative activity

doi: 10.1039/d6ra03557d

Figure Lengend Snippet: Selected thiazole/chalcone-based tubulin polymerization inhibitors (I–VII) and combretastatin A-4.

Article Snippet: The effects of compounds 9a–o on tubulin polymerization were assessed using a fluorescence-based tubulin polymerization assay (Cytoskeleton, Inc., Cat. No. BK011P), with combretastatin A-4 as the reference inhibitor.

Techniques:

Journal: RSC Advances

Article Title: Design, synthesis, and mechanistic evaluation of novel pyrazole/thiazole chalcone hybrids as dual tubulin polymerization and COX-2 inhibitors with potent antiproliferative activity

doi: 10.1039/d6ra03557d

Figure Lengend Snippet: Tubulin polymerization inhibitory activity of compounds 9a–o and the reference inhibitor CA-4, expressed as IC 50 values (µM), as determined from a fluorescence-based polymerization assay monitored kinetically for 60 min at 37 °C. Data are presented as mean ± SEM.

Article Snippet: The effects of compounds 9a–o on tubulin polymerization were assessed using a fluorescence-based tubulin polymerization assay (Cytoskeleton, Inc., Cat. No. BK011P), with combretastatin A-4 as the reference inhibitor.

Techniques: Activity Assay, Fluorescence, Polymerization Assay

Journal: RSC Advances

Article Title: Design, synthesis, and mechanistic evaluation of novel pyrazole/thiazole chalcone hybrids as dual tubulin polymerization and COX-2 inhibitors with potent antiproliferative activity

doi: 10.1039/d6ra03557d

Figure Lengend Snippet: Superimposition of the co-crystallized (green) and redocked (brown) colchicine poses within the colchicine-binding site of tubulin (PDB ID: 4O2B).

Article Snippet: The effects of compounds 9a–o on tubulin polymerization were assessed using a fluorescence-based tubulin polymerization assay (Cytoskeleton, Inc., Cat. No. BK011P), with combretastatin A-4 as the reference inhibitor.

Techniques: Binding Assay

Journal: RSC Advances

Article Title: Design, synthesis, and mechanistic evaluation of novel pyrazole/thiazole chalcone hybrids as dual tubulin polymerization and COX-2 inhibitors with potent antiproliferative activity

doi: 10.1039/d6ra03557d

Figure Lengend Snippet: Binding interactions of compound 9l within the colchicine-binding site of tubulin (PDB ID: 4O2B): (A) 2D interaction diagram and (B) 3D binding mode.

Article Snippet: The effects of compounds 9a–o on tubulin polymerization were assessed using a fluorescence-based tubulin polymerization assay (Cytoskeleton, Inc., Cat. No. BK011P), with combretastatin A-4 as the reference inhibitor.

Techniques: Binding Assay